Acetic Acid Solution 1%

Acetic Acid Solution 1%

Acetic Acid Solution 1% is a research-grade aqueous solution of glacial acetic acid diluted to 1% v/v in sterile water. It is widely used in peptide and protein research as a reconstitution solvent, particularly for lyophilised peptides that exhibit poor solubility in water alone. The mild acidity of a 1% acetic acid solution (pH ~3.4) aids in dissolving hydrophobic or aggregation-prone peptides without denaturing their structure.

Mechanism of Action

Acetic acid (C₂H₄O₂, MW 60.05 g/mol, CAS 64-19-7) is a simple carboxylic acid that protonates basic residues on peptide chains, increasing their net positive charge and improving aqueous solubility. At 1% v/v concentration, it provides sufficient acidity to solubilise most research peptides while remaining compatible with downstream dilution into physiological buffers.

Research Applications

• Reconstitution of lyophilised research peptides (e.g. IGF-1, GH fragments)
• Preparation of peptide stock solutions for in vitro assays
• Buffer preparation and pH adjustment in laboratory protocols
• Protein solubilisation and stability studies

Usage Notes

For peptide reconstitution, add a small volume of 1% acetic acid to the lyophilised peptide, allow to dissolve fully, then dilute to the desired concentration with sterile PBS or bacteriostatic water. Reconstituted solutions should be aliquoted and stored at -20°C to minimise freeze-thaw degradation.

Specifications

• Molecular Formula: C₂H₄O₂
• Molecular Weight: 60.05 g/mol
• CAS Number: 64-19-7
• Concentration: 1% v/v
• Form: Aqueous solution
• Storage: 4°C
• Shelf Life: 24 months

This product is intended for legitimate laboratory and analytical research use only. Not for diagnostic, therapeutic, or consumer use.