For research and laboratory use only. Not intended for human or veterinary use.
Reconstitution Guide
Materials Required
- Lyophilised peptide vial
- Bacteriostatic Water (BW) — preferred for most peptides
- 0.6% Acetic Acid Solution — for poorly soluble peptides (e.g. IGF-1 LR3, HGH Fragment)
- Sterile syringes and needles
- Alcohol swabs
- Refrigerator (2–8°C) or freezer (−20°C) for storage
Choosing the Right Solvent
- Bacteriostatic Water — suitable for BPC-157, TB-500, Semaglutide, Tirzepatide, CJC-1295, Ipamorelin and most GH secretagogues.
- 0.6% Acetic Acid — recommended for IGF-1 LR3, IGF-DES, and HGH Fragment 176-191.
- Sterile Water — short-term use only. Significantly reduces shelf life.
Reconstitution Protocol
- Allow the vial to reach room temperature — 10–15 minutes before opening.
- Prepare your solvent — draw the required volume into a sterile syringe.
- Clean the vial septum — wipe with an alcohol swab, air dry 30 seconds.
- Inject solvent slowly down the side of the vial — never directly onto the lyophilised cake.
- Do not shake — gently swirl until fully dissolved.
- Inspect the solution — should be clear and colourless. Discard if cloudy or particulate.
Calculating Concentration
Concentration (mg/mL) = Peptide mass (mg) ÷ Volume of solvent added (mL)
Example: A 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL.
Storage Guide
Lyophilised (Freeze-Dried) Peptides
- Temperature: Store at −20°C as standard. For long-term archival of sensitive peptides (e.g. IGF-1 LR3, Epithalon), −80°C is preferred.
- Moisture: Keep vials tightly sealed. Allow to reach room temperature before opening to prevent condensation.
- Light: Store in the dark or in amber vials. UV exposure causes oxidation of methionine and tryptophan residues.
- Desiccant: Store with silica gel packets to absorb residual moisture.
Reconstituted Peptides
- Bacteriostatic Water: Store at 2–8°C for up to 28 days.
- Sterile Water: Use within 5–7 days at 2–8°C.
- Acetic Acid Solution: Use promptly or aliquot and freeze.
Freeze-Thaw Cycles
Repeated freeze-thaw cycles are a primary cause of peptide degradation. Each cycle introduces mechanical stress and can cause aggregation or denaturation.
- Aliquot into single-use volumes before freezing.
- Limit freeze-thaw cycles to a maximum of 2–3.
- Never refreeze a thawed aliquot used in an assay.
Storage Summary
| Condition | Lyophilised | Reconstituted (BW) | Reconstituted (Sterile Water) |
|---|---|---|---|
| Temperature | −20°C | 2–8°C | 2–8°C |
| Stability | 24+ months | Up to 28 days | 5–7 days |
| Freeze-thaw | N/A | Minimise (≤3) | Avoid |
All products supplied by Vanta Labs are intended strictly for laboratory and research purposes.