Introduction
Lyophilised (freeze-dried) peptides must be reconstituted before use in laboratory assays. Correct reconstitution technique is critical to preserving peptide integrity, ensuring accurate concentration, and maintaining stability during storage.
For research and laboratory use only. Not intended for human or veterinary use.
Materials Required
- Lyophilised peptide vial
- Bacteriostatic Water (BW) — preferred for most peptides
- 0.6% Acetic Acid Solution — for poorly soluble peptides (e.g. IGF-1 LR3, HGH Fragment)
- Sterile syringes and needles
- Alcohol swabs
- Refrigerator (2–8°C) or freezer (−20°C) for storage
Choosing the Right Solvent
- Bacteriostatic Water — suitable for BPC-157, TB-500, Semaglutide, Tirzepatide, CJC-1295, Ipamorelin and most GH secretagogues.
- 0.6% Acetic Acid — recommended for IGF-1 LR3, IGF-DES, and HGH Fragment 176-191.
- Sterile Water — short-term use only. Significantly reduces shelf life.
Reconstitution Protocol
- Allow the vial to reach room temperature — 10–15 minutes before opening.
- Prepare your solvent — draw the required volume into a sterile syringe.
- Clean the vial septum — wipe with an alcohol swab, air dry 30 seconds.
- Inject solvent slowly down the side of the vial — never directly onto the lyophilised cake.
- Do not shake — gently swirl until fully dissolved.
- Inspect the solution — should be clear and colourless. Discard if cloudy or particulate.
Calculating Concentration
Concentration (mg/mL) = Peptide mass (mg) ÷ Volume of solvent added (mL)
Example: A 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL.
Storage After Reconstitution
- Bacteriostatic water: 2–8°C for up to 28 days.
- Longer storage: aliquot and freeze at −20°C. Avoid repeated freeze-thaw cycles.
- Lyophilised (unreconstituted): stable 24+ months at −20°C, kept dry and away from light.
All products supplied by Vanta Labs are intended strictly for laboratory and research purposes.